Case 2: Pharmaceutical Protein Production
During pharmaceutical protein production by use of Bioneer’s P170 expression system in Lactococcus lactis lactate is co-produced. Lactate inhibits the microbial growth, hence the production of recombinant protein is inhibited.
Studies show that REED connected to fermentation of Lactococcus lactis can increase the yield of recombinant protein significantly. REED removes lactate continously during fermentation and enables prolonged growth of the microorganisms.
In a Proof of Concept study L-arabinose isomerase (araA), an enzyme used to convert sugar to low-calorie sweetener, is produced by Lactococcus lactis. The yield was increased approximately 6 fold from 100 mg/L to 600 mg/L by connecting the REED system to the fermentation production of araA.
Read more cases about yield improving recombinant protein production here.
Later optimization of araA production in 15 L REED-fermentation showed an even higher productivity, see figure below. The trials were performed with two different anion exchange membranes, AR103 from Ionics (US) and Neosepta ASM from Astom (JP).